Abstract 142: Remote Ischemic Postconditioning Alone and Combined with Mild Hypothermia Improves Myocardial Function in a Porcine Model of Cardiopulmonary Resuscitation
Background: Current studies have demonstrated that remote ischemic post-conditioning (RIpostC) protects the heart during acute myocardial infarction. Cardiac arrest is a systemic ischemia injury. The present study investigated whether RIpostC alone and combined with mild hypothermia following resuscitation would provide an improvement of the myocardial function in a pig model of CPR.
Hypothesis: RIpostC following resuscitation would improve the myocardial function after cardiac arrest. Furthermore, RIpostC combined with mild hypothermia following ROSC would be more effective for improving post resuscitation myocardial function when compared with RIpostC only.
Methods: In 21 male domestic pigs weighing between 35-40 kg, ventricular fibrillation was induced and untreated for 10 mins. Defibrillation was attempted after 5 mins of CPR. The resuscitated animals were then randomized into 3 groups: Control, RIpostC or RIpostC combined with mild hypothermia (RIpostC+Cooling). Coincident with the start of CPR, RIpostC was induced by four cycles of 5 mins of limb ischemia and then 5 mins of reperfusion. At 5 mins after resuscitation, mild hypothermia was implemented by surface cooling to reach a temperature of 32-34°C until 4 hrs post-resuscitation. Global ejection fraction, stroke volume and NT-proBNP were measured at baseline and at hourly intervals for 4 hrs following resuscitation.
Results: Post-resuscitation global ejection fraction, stroke volume and NT-proBNP were significantly better in the animals treated with RIpostC when compared to the control group. However, significantly better post-resuscitation myocardial function was observed in the RIpostC+Cooling group than the RIpostC group (Table 1).
Conclusions: In a porcine model of CPR, RIpostC provided an improvement of post-resuscitation myocardial function. Furthermore, RIpostC combined with mild hypothermia would be more effective for protecting post-resuscitation myocardial function.
Author Disclosures: S. Ye: None. J. Xu: None. Z. Li: None. M. Wang: None. G. Chen: None. Z. Wang: None. W. Tang: None.
- © 2014 by American Heart Association, Inc.