Abstract 13136: MiR-320 as Potential Biomarker of Arrhythmogenic Right Ventricular Cardiomyopathy
Rationale: Diagnosis of Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) is challenging and often late after disease onset. MicroRNAs (miRNAs) have been proposed as circulating biomarkers of cardiac diseases such as heart failure, myocardial infarction and atrial fibrillation. No previous studies did evaluate the involvement of miRNAs in ARVC.
Aim: Our objective was to study circulating miRNAs expression profile in ARVC and correlate results in patient’s cardiac cells.
Methods: The expression of 367 miRNAs was screened in plasma samples from ARVC patients and matched healthy donors using TaqMan Arrays (n=3 symptomatic ARVC patients vs n=3 healthy subjects). Selected miRNAs were validated in the plasma of healthy subjects (n=15) and of patients with a diagnosis of ARVC as defined by current guidelines (n=14). Selected miRNA expression was then tested in cardiac stromal cells (CSCs) obtained from ventricular biopsies of ARVC and non-ARVC patients (n=3 each).
Results: Among 118 miRNAs found expressed in screened plasma samples, miR-320 showed a statistically significant down-regulation in ARVC patients (fold change 0.45±0.06, p=0.013). In addition, ROC analysis showed an area under the curve value of 0.76 (p=0.016), indicating a good accuracy of miR-320 as a possible marker of ARVC. A similar reduction of miR-320 expression was observed in CSCs obtained from right ventricle biopsies of ARVC patients vs non-ARVC controls (n=3, fold change 0.42±0.08, p=0.04). Intriguingly, miR-320 regulates WNT pathway, which is involved in ARVC pathogenesis.
Conclusions: mir-320 is significantly down-regulated in ARVC patient’s plasma, potentially representing a novel circulating early biomarker of ARVC. The down-regulation observed in ARVC CSCs and its involvement in the WNT pathway regulation may also suggest a mechanistic role in disease pathogenesis.
Author Disclosures: E. Sommariva: None. Y. D’Alessandra: None. S. Brambilla: None. F. Cattaneo: None. M. Casella: None. M.C. Capogrossi: None. C. Tondo: None. G. Pompilio: None.
- © 2014 by American Heart Association, Inc.