Abstract 13111: Determinant of Sucessful Implantation of a Novel Autologous Valve Substitute (Biovalve) with Transcatheter Technique
Introduction: There are some attempts to use tissue-engineered materials for heart valvular substitutes, but it is not clear what is determinant of successful implantation of the substitutes. It is generally thought that appropriate cell migration into an implanted graft tissue is essential in order to sufficiently exert the functions as a native valve.
Hypothesis: We are developing a novel autologous aortic valve (Biovalve), using simple, safe and economical in-body tissue engineering which is based on a tissue encapsulation phenomenon in the living body. In this study, we investigated key factors in hitogenesis when the Biovalve was implanted in a large animal model with transcatheter technique.
Methods: Biovalves were prepared by 2-month embedding of the molds, assembled using plastic rods in the subcutaneous spaces of goats. After extracting the molds and removing the plastic rods only, Biovalve were constituted from completely autologous connective tissues. We combined the Biovalve with a metaric stent for transcatheter implantation. Twenty Biovalve Stents were implanted in the aorta (n=16) and the pulmonary artery (PA) (n=4) in situ with transcatheter apical approach technique.
Results: The Biovalve Stents were successfully implanted in both aortic and PA postion. They were extracted every month (maximum 6 months) after implantation to observe their cell migration process. The leaflets of the Biovalve kept their shape and elasticity even after 6 months and neither calcification nor thrombi were observed. Histological examination showed the cell migration inside the Biovalve body and laminar endothelialization on the surface of the valve leaflets even in 1 month after implantation. Their histogenesis have gradually advanced in 6 months without any hyperplasia, but were interrupted when the Biovalve tissues were not attached enough on the native vascular wall surface.
Conclusions: The Biovalve satisfied the higher requirements of systemic and pulmonary circulation in goats for 6 months with the histogenic potential that was mainly induced by cell migration from the native vascular tissues contacting with the Biovalve.
Author Disclosures: Y. Takewa: None. Y. Nakayama: None. S. Kishimoto: None. K. Date: None. H. Sumikura: None. K. Kanda: None. T. Tajikawa: None. T. Tanaka: None. Y. Taenaka: None. E. Tatsumi: None.
- © 2014 by American Heart Association, Inc.