Abstract 12802: Western Diet Impairs Cardiac Systolic and Diastolic Function in the Mouse
Introduction: Western diet (WD), rich in saturated fat and sucrose, has pro-inflammatory properties and promotes cardiovascular disease. Whether WD has direct effects on cardiac function is unknown.
Hypothesis: We hypothesized that WD would negatively affect cardiac systolic and diastolic function in the mouse.
Methods: CD1 8 week-old male mice were fed with standard chow diet with 17% fat content (N=8) or WD with 42.0% fat content (N=11) for 8 weeks. Food intake was measured daily. Mice underwent transthoracic echocardiography under mild sedation with pentobarbital at baseline, 4 weeks and 8 weeks to measure left ventricular end-diastolic diameter (LVEDD), ejection fraction (LVEF), mass (LVM), myocardial performance index (MPI) and isovolumetric relaxation time (IVRT). Left ventricular catheterization through retrograde right carotid artery approach was performed with a Millar catheter to measure peak systolic pressure (LVPSP) and end-diastolic pressure (LVEDP). An additional group of female mice (N=13) was followed for 4 weeks with WD.
Results: When compared to baseline, WD induced a progressive time-dependent impairment in LVEF (-5% at 4 weeks and -12% at 8 weeks [P=0.002], Figure), which was directly correlated with the amount of food intake (R=-0.36, p=0.041), without changes in LVEDV or LVM or LVPSP. A significant increase in MPI (+84%, P=0.003) and in the IVRT (+75%, P=0.047), reflecting impaired diastolic function, was seen already at 4 weeks, preceding changes in LVEF (Figure), and it was associated with a significant increase in the LVEDP (8 vs 3 mmHg, P=0.034). Similar effects on the LVEF (-7%, p=0.04) were seen in female mice after 4 weeks of WD.
Conclusions: Western diet, rich in saturated fat and sucrose, impairs cardiac systolic and diastolic function in the mouse. The mechanisms underlying diet-induced effects on cardiac function impairment, and whether diet contributes to the syndrome of heart failure in patients require further exploration.
Author Disclosures: S. Carbone: None. S. Toldo: None. A. Nordio: None. C. Marchetti: None. E. Mezzaroma: None. A.G. Mauro: None. J. Regan: None. C. Sonnino: None. F.N. Salloum: None. B.W. Van Tassell: None. A. Abbate: Research Grant; Modest; Grifols. Other Research Support; Modest; Swedish Orphan Biovitrum. Research Grant; Significant; Novartis.
- © 2014 by American Heart Association, Inc.