Abstract 12739: Different Expression in Targeted Metabolomic Study Between Normothermia and Hypothermia Treatment After Cardiac Arrest
Introduction: Therapeutic hypothermia is the most effective treatment for the post-cardiac arrest myocardial dysfunction and can improve the long-term survival. However, the mechanism of its protective effects remained unclear. Metabolomics study is a way of measuring and analyzing the small molecular metabolites of whole body in different physiological or pathological conditions.
Hypothesis: Investigate the changes of metabolites after cardiac arrest and resuscitation and the difference of expression profiles between hypothermia and normothermia post-cardiac arrest care.
Methods: Cardiac arrest was induced by asphyxia 8.5 mins in male adult Wistar rats. Animals were randomized to hypothermia with targeted temperature 32oC or normothermia treatment without temperature control after ROSC (n=10 for each group). Blood specimens were sampled at time points of pre-arrest, ROSC and 1 hour after cardiac arrest. The levels of metabolites were presented as the ratio to the pre-arrest status. Targeted metabolites analyses were applied for the samples.
Results: After cardiac arrest and resuscitation, the ratio of circulating acetyl-carnitine level at ROSC to pre-arrest status increased to 20.81±0.16 (P<0.01). The ratio of circulating alanine level at ROSC to pre-arrest status increased to 1.95±0.45 (P<0.01). The ratio of circulating glutamine level at ROSC to pre-arrest status increased to 2.01±1.03 (P<0.01). Under hypothermia treatment in post-cardiac arrest phase, the ratio of circulating threnonine level increased from 1.22±0.15 to 1.43±0.14 compared to the normothermia group (P=0.05). In the other way, the ratio of circulating trimethylamine-N-oxide (TMNO) level decreased from 1.43±0.57 to 0.94±0.30 (P=0.03) with hypothermia treatment.
Conclusions: Injury-related metabolites with increasing of threonine and decreasing of TMNO are modified under hypothermia treatment.
Cardiac arrest is associated with diverse, but incompletely understood metabolic disturbances. Metabolomic approaches can provide higher resolution phenotyping of these disturbances. Understanding the functional signi[[Unable to Display Character: ﬁ]]cance of selected [[Unable to Display Character: ﬁ]]ndings is important in the future.
Author Disclosures: C. Huang: None. M. Tsai: None. W. Chang: None. C. Kuo: None. W. Chen: None.
- © 2014 by American Heart Association, Inc.