Abstract 12731: MicroRNAs 196b-5p and 411-5p Are Associated With Post-Operative Atrial Fibrillation
Introduction: Post-operative atrial fibrillation (POAF) develops in 1/3 of patients undergoing cardiac surgery and is associated with significant morbidity and mortality. MicroRNAs (miRs) are gene regulators linked to pathological atrial remodeling. Although genetic factors are implicated in susceptibility to POAF, no studies, to our knowledge, have examined atrial miR expression in relation to POAF.
Hypothesis: Changes in miR expression (estimated as fold difference in the delta cycle threshold compared to global mean) in human atria can be associated with POAF.
Methods: Atrial tissue (26 from right atrium; 2 from left atrium) was obtained from 28 participants with no history of atrial fibrillation undergoing elective cardiac surgery (36% coronary artery bypass grafting, 50% valve replacement and 14% combined surgeries or others). Based on pilot data and prior literature, the expression of 82 miRs was quantified using high-throughput quantitative reverse-transcriptase polymerase chain reaction. We used logistic regression adjusted for age and sex to detect the associations between atrial miRs and POAF.
Results: The mean age of the cohort was 68 years (±11) and 75% were men. A history of coronary artery disease and heart failure was present in 54% and 29% respectively. POAF developed in 57% within a follow-up of 1 month. Among patients with POAF, the age- and sex-adjusted odds ratio for miR 196b-5p expression was 28 (p = 0.02) and that for miR 411-5p was 0.05 (p = 0.04) compared to those with no POAF. The fold difference in miRs 196 and 411 in patients with POAF is 0.29 and -0.43, respectively, compared to those with no POAF.
Conclusion: In our study, the expression of miR 196 was higher while that of miR 411 was lower in those with POAF. These miRs are known to control expression of genes regulating apoptosis, inflammation and ion channel function. Our novel results suggest that miRs 196 and 411 are associated with POAF and may identify surgical patients at risk for this arrhythmia.
Author Disclosures: M. Kinno: None. N. Esa: None. R.S. Velagaleti: None. A.Y. Shaikh: None. K. Tanriverdi: None. H. Lin: None. D. Mandapati: None. S. Tam: None. O.N. Okike: None. J.F. Keaney, Jr.: None. J.E. Freedman: None. J. Donahue: None. D.D. McManus: None.
- © 2014 by American Heart Association, Inc.