Abstract 12004: Correction of Hyperphosphosphatemia by Dietary Phosphorus Restriction or Phosphorus Binder Similarly Ameliorates Vascular Complications and Mineral Disorders in CKD Rats
Introduction: Hyperphosphatemia has been identified as an independent risk factor for cardiovascular disease (CVD) in chronic kidney disease patients. Higher serum phosphorus (P) concentration can cause both endothelial dysfunction and vascular calcification. Dietary P restriction and P binders are available for correction of hyperphosphatemia, however, it has not been clarified if both treatments can similarly ameliorate vascular complecations, especially endothelial dysfunction, as well as mineral disorders in CKD patients or not.
Hypothesis: Here, we compared lanthanum carbonate as a P binder with dietary P restriction on the correction of hyperphosphatemia and endothelial dysfunction in adenine-induced chronic kidney disease rats.
Methods: Adenine-induced CKD model rats were prepared by feeding 0.5% adenine containing diet for 35 days on male 7-wk-old Sprague-Dawley rats. Then, the adenine-induced CKD rats were divided 5 groups and treated with either control diet (CP; 1%P), low P diet (LP; 0.2%P), 1.5% lanthanum carbonate (La1.5; 1%P, 1.5%LaCO3), 3% lanthanum carbonate (La3; 1%P, 3%LaCO3), or 6% lanthanum carbonate (La6; 1%P, 6%LaCO3) for 14 days. After sacrifice, plasma P, intact-PTH, FGF23, and creatinine were measured. We evaluated acetylcholine-dependent vasodilation of aortic ring by isometric transducers, and F4/80 positive cells and MCP-1 expression on aortic arch by immunohistochemical analysis.
Results: Hyperphosphatemia, high PTH and FGF23 levels were similarly and significantly corrected in LP and La6 groups compared with CP group. LP and La6 groups similarly and significantly ameliorated impaired vasodilation in response to acetylcholine. However, correction of hyperphosphatemia was not enough in La1.5 and La3 groups so that impaired vasodilation was not significantly ameliorated. LP and La6 groups also decreased F4/80 positive cells and MCP-1 expression on aortic arch.
Conclusions: Correction of hyperphosphatemia by either dietary P restriction or lanthanum carbonate can similarly ameliorate vascular complecations and mineral disorders including abnormal PTH and FGF23 observed in adenine-induced CKD rats.
Author Disclosures: Y. Taketani: Research Grant; Significant; Bayer Yakuhin, Ltd. H. Ueda: None. M. Abuduli: None. H. Yamamoto: None.
- © 2014 by American Heart Association, Inc.