Abstract 11919: AMP-Activated Protein Kinase Response to Metabolic Stress: A Novel Determinant of Atrial Fibrillation Progression
Introduction: Metabolic stress is observed in clinical and experimental atrial fibrillation (AF). Metabolic stress activates AMP-dependent protein kinase (AMPK) via phosphorylation; activated AMPK produces compensatory changes in the determinants of energy balance. Here, we examined AMPK responses in AF and their role in remodeling responses of atrial Ca2+ handling and contractility.
Methods: AMPK and Cav1.2 protein were quantified by immunoblot. Ca2+ transients (CaTs, Indo1 AM), cell shortening (CS, videometry), L-type Ca2+ (ICa,L) and NCX (INCX) current (patch clamp) were measured in dog left atrial (LA) cardiomyocytes (CMs) under metabolic stress due to glycolysis inhibition (GI, 10 mM 2-deoxyglucose/10 mM pyruvate).
Results: In dogs with 1-wk electrically-maintained AF (n=4), the phosphorylation ratio (PhR) of AMPK (indicating activation) increased in LA by 101%* (*p<0.05) vs. controls (n=4). Metabolic stress due to GI in 2 Hz paced LA CMs increased AMPK PhR by 103%* vs. quiescent CMs. In 2 Hz paced cells, the AMPK inhibitor CompC (10 μM) decreased LA CaT and CS by 58%* and 51%* vs. control, but the AMPK activator AICAR (1 mM) restored CaT and CS by 75%* and 53%* vs. CompC alone, supporting AMPK-dependent regulation. CompC decreased ICa,L and ICa,L-triggered CaT by 46%* and 37%*. AMPKα protein coimmunoprecipitated with Cav1.2 protein, suggesting that AMPK is physically coupled with the ICa,L channel α-subunit. CompC also decreased SR Ca2+ content by 53%* vs. control (INCX simultaneously measured with caffeine-induced CaT). AMPK PhR was greater by 61%* in paroxysmal AF (pAF, n=7) patient atria vs. sinus rhythm controls (n=10), whereas AMPK PhR was decreased in chronic AF (cAF) patients (n=9) by 27%*, suggesting that the level of AMPK activation may govern the chronicization of AF.
Conclusions: AF activates AMPK. Under AF-induced metabolic stress, AMPK activation limits AF-promoting abnormalities in LA ICa,L, Ca2+ handling and contractility. pAF patients have AMPK activation, protecting them against chronicization, whereas cAF patients have reduced AMPK activation. Thus, the response of AMPK to AF-related metabolic stress might be a crucial determinant of AF chronicization, and AMPK-manipulation may be a novel target to prevent AF progression.
Author Disclosures: M. Harada: None. X.Y. Qi: None. A. Tadevosyan: None. N. Voigt: None. M. Karck: None. M. Kamler: None. I. Kodama: None. T. Murohara: None. D. Dobrev: None. S. Nattel: None.
- © 2014 by American Heart Association, Inc.