Abstract 11525: Blood Pressure Reduction and Increase of Circulating MicroRNA-126 Indicate Improvement of Vascular Endothelial Damage After Smoking Cessation Intervention in Middle-Aged Habitual Smokers
Introduction: Cigarette smoking promotes vascular endothelial damage and accelerates progression of atherosclerosis. The purpose of this study was (1) to examine effects of smoking cessation intervention on vascular function and (2) to investigate whether circulating level of microRNA-126 (miR-126), a vascular endothelium-enriched microRNA, would recover from smoking-related endothelial damage in middle-aged habitual smokers.
Methods: Middle-aged male smokers (n=30) were enrolled and instructed to stop smoking. Their clinical profiles of vascular function and laboratory findings including serum levels of cotinine, a principal metabolite of nicotine, and expression of miR-126 were investigated at baseline and 8 weeks after the instruction. Vascular function for peripheral small vessels was assessed using End-PAT 2000 and expressed as reactive hyperemia peripheral arterial tonometry (RH-PAT) index. The expression of miR-126 in plasma was analyzed by quantitative real-time PCR.
Results: At baseline, serum cotinine levels were inversely correlated with vascular function expressed as RH-PAT index (r=-0.48, P<0.01) and positively correlated with levels of metabolic parameters such as non-HDL cholesterol (r=0.53, P<0.01) and HOMA-IR (r=0.52, P<0.01). More than half of the smokers (n=17) could not completely attain smoking cessation. However, changes in the RH-PAT index showed a significant correlation with those in systolic blood pressure (r=-0.54, P<0.01). Moreover, the RH-PAT index was significantly increased in subjects who showed a reduction in systolic blood pressure (1.83±0.29 to 2.24±0.56, P<0.05). In contrast, smokers who completely attained smoking cessation (n=13) showed significant increase in RH-PAT index and plasma levels of miR-126 (P<0.05, respectively).
Conclusions: Peripheral vascular function was improved 8 weeks after the instruction of smoking cessation in subjects who showed a blood pressure reduction. Subjects with complete smoking cessation exhibited increase in circulating miR-126. These findings suggest that blood pressure reduction and increase of miR-126 may be a key process to achieve an improvement of vascular function from smoking-related endothelial damage.
Author Disclosures: T. Sugiura: None. Y. Dohi: None. S. Yamashita: None. A. Sanagawa: None. S. Iwaki: None. N. Ohte: None. S. Fujii: None.
- © 2014 by American Heart Association, Inc.