Abstract 11199: Impact of Rosuvastatin Treatment on Reduction of Thrombus Burden in Rat Acute Inferior Vena Cava Constriction
Background: This study tested the hypothesis that rosuvastatin reduces thrombus burden through inhibiting inflammation and suppressing reactive oxygen species (ROS) generation in an inferior vena cava Constriction (IVCCO)-induced deep vein thrombosis (DVT) rat model.
Methods and Results: 12-week-old male Sprague-Dawley rats (n=24) were equally divided into sham control (group 1: laparotomy only), IVCCO (group 2: IVC constriction), and IVCCO + rosuvastatin (20 mg/kg/day, orally after induction of IVC constriction) (group 3). IVC diameter was measured by days 0 and 14 and the right hindlimb thickness was measured by day 0, 7, and 14 prior to scarifying the animals. The results showed significantly increased IVC diameter and hindlimb thickness in group 2 than in groups 1 and 3, and significantly increased in group 3 than in group 1 by day 14 after the procedure (all p<0.001). Additionally, WBC count and prevalence of helper T cells, cytotoxic T cells, regulatory T cells, and early and late apoptotic mononuclear cells (MNCs) in circulation were significantly higher in group 2 than in group 1, and were significantly suppressed in group 3 after treatment (all p<0.001). Furthermore, inflammation at cellular (CD68+ cells) and protein (MMP-9, TNF-α) levels, oxidative stress (oxidized protein) and reactive oxygen species (NOX-1, NOX-2) in IVC also showed similar changes as those of immune cells in circulation among the three groups (all p<0.01).
Conclusion: Rosuvastatin treatment significantly reduced IVC thrombus burden through inhibiting inflammatory response and oxidative stress in a rodent model of DVT.
Key words: inferior vena cava constriction, deep vein thrombosis, rosuvastatin, inflammation, oxidative tress, reactive oxygen species
Author Disclosures: S. Chua: None. J. Sheu: None. S. Leu: None. T. Tsai: None. H. Yip: None.
- © 2014 by American Heart Association, Inc.