Abstract 9786: Wnt11 Regulates Angiogenesis Through a Non-canonical Wnt-PKC-JNK Pathway
Wnt11 has been implicated in stem cell proliferation and differentiation. However, its role in angiogenesis remains largely unknown. We investigated angiogenic role of Wnt11 on human vascular endothelial cells (HUVECs) and documented its related signaling pathways. HUVECs pre-labeled with PKH26 were seeded in BD Matrigel Matrix (growth factor reduced) at 1.5х104 /cm2 and treated with recombinant Wnt11 proteins at 5μg/ml. Both the length and the number of capillary-like tubes (CLT) were measured after HUVECs were treated for 18 hours. Wnt11 significantly increased the number and length of CLT (Fig. A). The migration of HUVECs assessed with the BD Biocoat™ Angiogenesis System was also promoted by Wnt11 (Fig. B). Immunostaining (Fig. C) and Western blotting (Fig. D) results revealed upregulation of p-pan-PKC and p-JNK in HUVECs transfected with Wnt11 (HWnt11) compared to control cells transduced with empty vector (HGFP). Moreover, the effect of Wnt11 on CLT formation and HUVECs migration were abrogated when the JNK inhibitor SP600125 (SP) at 5 μM and the PKC inhibitor Calphostin-C (Cal-C) at 0.1 μM were added to the culture system (Fig. E and F). Our data suggested, for the first time, that Wnt11 promotes endothelial cell migration and capillary formation, which is related to the activation of the non-canonical Wnt-PKC-JNK pathways.
- © 2013 by American Heart Association, Inc.