Abstract 9444: Study of Natriuretic Peptides Levels in the Left Ventricular Tissue of Patients With Dilated Cardiomyopathy Using RNA-Seq
Background: Natriuretic peptides (NPs) levels have been extensively studied in plasma of heart failure (HF) patients. Currently, BNP and proBNP are used for diagnosis and prognosis. Our objective is to evaluate the tissue levels of these peptides and several molecules involved in their processing and biological activity at mRNA level, in the left ventricle of patients with dilated cardiomyopathy (DCM) undergoing heart transplantation.
Methods: We analyze the mRNA levels of 14 left ventricular samples from patients with nonischemic DCM undergoing heart transplantation and 7 control subjects (CNT), using massive sequencing, RNAseq, (SOLID 5500XL) and RT-qPCR (ViiATM 7 Real-Time PCR System).
Results: ANP and BNP tissue levels are elevated in DCM compared with CNT group (fold change (FC)=37, p<0.01 and FC=26, p<0.05, respectively). CNP was not detected by RNAseq in the left ventricular samples of patients with DCM or in CNT group. NPs receptors (NPR1, NPR2 and NPR3) show no significant differences in mRNA levels between the two study groups. Finally, regarding the enzymes involved in the cleavage of these peptides we observed that corin is significantly decreased in the DCM group compared to CNT (FC=-2.5, p<0.01), while no significant changes are observed in furin.
Conclusions: RNA-Seq analysis confirm the presence of ANP in adult ventricular tissue, suggesting that the elevations observed in plasma could be originated in part in the left ventricle. CNP was not detected in cardiac tissue. Changes are specific since there were no modifications in the levels of any of the receptors and only in one of the enzymes involved.
- © 2013 by American Heart Association, Inc.