Abstract 196: Remote Ischemic Pre-Conditioning Improves Microcirculation following Severe Hemorrhagic Shock in a Rat Model
Introduction: The microcirculatory flow is a major determinant of tissue oxygen delivery. A significantly reduced microcirculatory flow has been described during and following hemorrhagic shock. We investigated whether remote ischemic pre-conditioning (RIPC) would improve microcirculation following severe hemorrhagic shock.
Hypothesis: RIPC improves sublingual microcirculation following hemorrhagic shock.
Methods: Fourteen male Sprague-Dawley rats were randomized into two groups: 1) Remote ischemic pre-conditioning (RIPC) group, 2) Control group. RIPC was performed by four cycles of 5 mins of limb ischemia followed by reperfusion for 5 mins. Hemorrhagic shock was induced by reduction of total blood volume by 50% over an interval of 1 hour, 30 mins after bleeding, reinfusion was initiated with the shed blood over the ensuing 30 mins. Sublingual microcirculation was measured by a sidestream dark-field imaging device at baseline, 1 hour after bleeding, 30 mins after shock, 30 mins after reinfusion and at hourly intervals thereafter for a total of 120 mins.
Results: The sublingual microvascular flow index (MFI) and perfused vessel density (PVD) were significantly reduced during hemorrhage (P<0.001) when compared with the baseline values. The MFI and PVD were significantly greater after reinfusion in the RIPC group than that in the Control group (P<0.001)(Table).
Conclusions: RIPC improved sublingual microcirculation following severe hemorrhagic shock.
- © 2013 by American Heart Association, Inc.