Abstract 18985: Lentil-Based Diets Reduce Vascular Remodeling Through a p38 Mitogen-Activated Protein Kinase Dependant Mechanism in the Spontaneously Hypertensive Rat
Hypertension is a major driving force behind arterial remodeling, which increases stiffness, and decreases compliance and function. Certain pharmaceuticals are capable of lowering high blood pressure, but most interventions are unable to also reverse remodeling. We have found that lentils are able to significantly attenuate the development of hypertension in the SHR, however the mechanism for this action remains unresolved. The current study examined the hypothesis that the positive actions of lentils on blood pressure are mediated through direct changes in arterial function that decrease remodeling and improve arterial compliance. Seventeen-week SHR were randomly assigned to groups (n=8/group) fed (i) 30% w/w green lentils, (ii) 30% w/w red lentils, (iii) 30% w/w mixed lentils (red and green), or (iv) no lentils. Normotensive WKY control rats were fed either mixed lentil or no lentil diets (n=8/group). BP and pulse wave velocity were measured weekly; pressure myography was performed at termination. Western Blotting was performed on aorta protein samples. There were no dietary related changes in pulse wave velocity, however, green lentils restored arterial compliance to a level better than control-fed WKY. All lentil fed animals exhibited arterial stiffness comparable to WKY control, with green lentils experiencing the largest improvement according to myography measurements. All lentils reduced the media:lumen ratio to a level between control fed SHR and WKY. Western blotting indicated a decrease in p38 MAPK activation in lentil-fed SHR with no change in ERK1/2 phosphorylation. Although both ERK1/2 and p38 MAPK are potent mediators of vascular remodeling, only p38MAPK phosphorylation is redox sensitive. These data would indicate that lentil-based diets are able to decrease vascular remodeling by ameliorating p38 MAPK-dependent redox-sensitive signaling cascades.
- © 2013 by American Heart Association, Inc.