Abstract 17801: The Effects of Resveratrol on Chronically Ischemic Myocardium in a Swine Model of Metabolic Syndrome: A Proteomics Study
Background: Resveratrol has been shown to improve cardiac perfusion and ventricular function after chronic ischemic injury. Using proteomic analysis we sought to objectively investigate potential mechanisms by which resveratrol exerts its cardio-protective effects in the setting of metabolic syndrome and chronic myocardial ischemia.
Methods and Results: Yorkshire swine were divided into two groups based on diet: high cholesterol (n= 7) or a high cholesterol diet with supplemental resveratrol (n= 6). Four weeks later all animals underwent surgical placement of an ameroid constrictor to their left circumflex artery. Diets were continued for another seven weeks then ischemic myocardium was harvested for proteomics analysis. Proteomic analysis identified 669 common proteins between the two groups. Of these proteins 76 were statistically different, of which 41 were characterized (p<0.05). Pathway analysis demonstrated that in animals supplemented with resveratrol there was a down-regulation in several proteins involved with mitochondrial dysfunction, cell death and unfavorable cardiac remodeling. Further, there was an up-regulation in proteins involved in free radical elimination.
Conclusions: Resveratrol supplementation significantly alters several critical protein markers in chronically ischemic myocardium. Further investigation of these proteins may help elucidate the mechanisms by which resveratrol exerts its cardioprotective effects.
- © 2013 by American Heart Association, Inc.