Abstract 173: Early Combined Cooling Improves the Cerebral and Cardiac Microcirculation in a Prolonged Cardiac Arrest and Cardiopulmonary Resuscitation Porcine Model
Objective: We previously reported that selective head cooling coincident with CPR could improve cerebral microcirculation and myocardial function. In the present study, we rapidly induced systemic mild hypothermia early after resuscitation to investigate this short duration cooling effect on microcirculation of brain and heart in a porcine model of prolonged cardiac arrest. We hypothesized that even two hours early combined cooling would improve cerebral and myocardial microcirculation.
Method: Ventricular fibrillation (VF)was induced electrically in 14 domestic pigs weighting 36±2 kg. After 11 minutes of untreated VF, CPR was performed manually for 6 minutes prior to defibrillation.
Coincident after got ROSC, animals were immediately randomized to receive combined cooling with 30 mL/kg 4°C saline and water blanket or keep normal temperature. In cooling group, animal’s temperature was induced to 32°C -34°C and maintained for 2 hours and then rewarmed to 38°C within another two hours. Cerebral and myocardial micro-circulation was monitored with the aid of colorful microsphere injected into left ventricle every two hours after ROSC.
Result: There is no significant differences of baseline, compression depth, CPP during CPR, total shocks time and dose of epinephrine in both groups. The cerebral microcirculation of animals in cooling group were significantly better than which of control group animals at 20min, 120min, 240min and 360min after ROSC (p < 0.05). The micro blood flow of endocardium in cooling group is higher than control group, but there are no differences between two groups.
Conclusion: In this model, even two-hour early combined cooling improves cerebral and myocardial microcirculation after resuscitation.
- © 2013 by American Heart Association, Inc.