Abstract 170: Remote Ischemic Post-Conditioning Improves the Outcomes of Cardiopulmonary Resuscitation in a Rat Model of Cardiac Arrest
Introduction: Remote ischemic post-conditioning (RpostC) has been proven to provide potent protection of vital organs against regional ischemia reperfusion injury. In this study, we investigated the effects of RpostC on the outcomes of CPR in a rat model of cardiac arrest.
Hypothesis: RpostC would reduce the severity of post-CPR myocardial dysfunction and prolong the duration of survival.
Methods: Ventricular fibrillation was induced and untreated for 6 mins in fourteen male Spague Dawley rats. The animals were then randomized to receive RpostC or sham control. Coincident with the beginning of CPR, RpostC was induced by four cycles of 5 mins of limb ischemia and then 5 mins of reperfusion. Defibrillation was attempted after 8 mins of CPR. The resuscitated animals were monitored for 4 hours and observed for an additional 68 hours.
Results: Five of the seven rats in each group were successfully resuscitated. Post-resuscitation myocardial function was significantly impaired in all animals. However, myocardial function gradually improved 4 hours after resuscitation and was significantly better in animals receiving RpostC (Table). All animals survived for 72 hours in the RpostC group. Significantly shorter duration of survival of 34±25 hours and significantly worse 72-hour survival rate was observed in the Control group.
Conclusion: In a rat model of cardiac arrest, RpostC significantly reduced the severity of post-resuscitation myocardial dysfunction and improved survival and neurological recovery.
- © 2013 by American Heart Association, Inc.