Abstract 15832: Increased mRNA Expression of Osteogenic Determinants in Epicardial Adipose Tissue of Patients With Severe Aortic Stenosis
Background and aims: Epicardial adipose tissue (EAT) is widely accepted to be an active endocrine and paracrine organ that influences key pathogenic mechanisms of atherogenesis. Recent studies have demonstrated that early atherosclerosis, cell proliferation, and calcifying phenotype are all hallmark features of aortic valve stenosis (AS). A potential relationship between EAT and the pathogenesis of calcified AS has never been explored. In this study we studied mRNA expression of osteogenic and inflammatory markers in the EAT of pts with calcified AS.
Methods: Total RNA was isolated from EAT biopsies obtained from pts operated for isolated severe calcified AS (n.8) and isolated coronary artery disease (CAD) (n.8). EAT was first characterized by analysis of the expression of adipocyte-specific gene Peroxisome proliferator-activated receptor γ. Next, mRNA levels of Collagen type 1 alpha 1 (COL1A1) and Bone Morphogenic Protein 1 (BMP1) were analyzed as osteogenic differentiation markers. Finally, Interleukin-8 (IL-8) and CCL5 mRNA expression levels were evaluated as inflammatory markers.
Results: Expression of osteogenic markers was significantly higher in pts with isolated AS as demonstrated by increased mRNA levels of COL1A1 and BMP1 (Figure). In contrast, EAT from pts with isolated CAD demonstrated a more pronounced inflammatory profile, as indicated by the higher leves of IL-8 and CCL5 (Figure).
Conclusions: Our preliminary results suggest that EAT might contribute to the calcifying phenotype of AS. Further, the increased mRNA expression of inflammatory markers in pts with CAD corroborates the correlation between EAT and atherogenesis.
Figure: AU= Arbitrary Units. Asterisks denote statistically significant values (* p < 0.05).
- © 2013 by American Heart Association, Inc.