Abstract 15791: Resistance Exercise Acutely Increases the Circulating Number of Endothelial Progenitor Cells
Introduction: The current evidence indicates that aerobic exercise acutely stimulates the mobilization of endothelial progenitor cells (EPCs) from the bone marrow, which may be sustained up to 2-3 days. Nevertheless, it is unknown, both in health and disease, whether other exercise modalities such as resistance training could influence the circulating number o EPCs.
Purpose: This study aimed to investigate the acute effects of a single bout of resistance exercise on the circulating number of EPCs.
Methods: Nine female university students (mean age: 21±1.66 years old; height: 1.63±0.47 meters; weight: 61±8.84 kg) volunteered to participate in this study. Subjects visited the lab twice, once for assessment of muscle strength to determine maximal resistance for each muscle group that was going to be exercised in the exercise session, and once (at least 72h after the first visit) for the exercise session. The resistance exercise session was performed at 80% of 1 maximum repetition and comprised 3 sets of 12 repetitions of the following exercises: bench press, dumbbell curl, dumbbell squat, and standing dumbbell upright row. The subjects rested 1 minute between sets and the session lasted approximately 30 minutes. Venous blood was collected at baseline, immediately after exercise, and 6 and 14 hours postexercise. To evaluate EPCs in the peripheral blood by flow cytometry, whole blood samples were labelled with monoclonal antibodies against CD34, CD309, and CD45. Identification of the EPCs was based on morphological properties and CD45-CD309+CD34+ profile.
Results: There was a statistically significant difference in circulating EPCs depending on the time of measurement, χ2(3)=12.978, p=0.005. Then, post-hoc analysis with Wilcoxon signed-rank tests indicated significant differences between EPCs at baseline [0.0075(0.0035)%] and immediately after exercise [0.0101(0.0048)%] (Z=-2.136, p=0.033), and 6h postexercise [0.0124(0.0083)%] (Z=-2.194, p=0.028). There was no significant increase in EPCs at 24h postexercise [0.0077(0.0055)%] compared to baseline (Z=-1.122, p=0.262).
Conclusions: Resistance exercise in healthy individuals leads to an acute increase in circulating EPCs that may be sustained up to 6 hours after the session termination.
- © 2013 by American Heart Association, Inc.