Abstract 14381: Ischemic but not Non-Ischemic Exercise Increases Stromal Derived Factor-1 Levels in Humans
Background: Therapeutic angiogenesis is being investigated as alternative therapy for myocardial ischemia. Angiogenesis is initiated in response to ischemia. Exercise training has been demonstrated to induce both angiogenesis and arteriogenesis in animal models. Human data relating the signaling of angiogenesis in response to ischemic exercise training is limited.
Methods: We studied 7 males (56±8y) with known coronary artery disease. All have angina pectoris, critical non-revascularized coronary artery stenosis and positive exercise stress test. Ischemic threshold was defined as heart rate at 1mm ST depression during stress test. All subjects underwent 3 acute bouts of acute exercise above the ischemic threshold one week apart and 1 bout of exercise below the ischemic threshold. The bouts of acute ischemic exercise included 5 min duration, 10 min duration, and 2 cycles of interval training at 1.5min on and 3.5min off conducted in random order. Vascular endothelial growth factor (VEGF) and stromal derived factor-1 (SDF) were measured by ELISA at baseline, immediately and 60min post exercise. As there were no differences between different types of acute ischemic exercises, the results were combined for analysis. ANOVA was used to compare the means between the different time points.
Results: With ischemic training, there were no significant change in VEGF levels. In contrast, SDF levels increase immediately post exercise and fell back towards baseline at one hour post. No changes were seen in either VEGF or SDF with non ischemic exercise (table).
Conclusion: we found that SDF levels increase only in response to exercise above ischemic threshold but not to exercise below ischemic threshold. These results have important implications in design of clinical studies of arterio- and angiogenesis.
- © 2013 by American Heart Association, Inc.