Abstract 14294: Enhanced Advanced Glycation End Product Receptors are Associated With Plaque Neovascularization and Inflammation in Peripheral Atheroma From Patients With Diabetes Mellitus
Background: Advanced Glycation End (AGE) product receptor expression in Diabetes Mellitus (DM) is associated with cellular and extra-cellular structural and functional changes in the vessel wall that may have pro-angiogenic and pro-inflammatory changes. However, presently no study exists to correlate neovascularization and inflammation in the Peripheral Arterial Disease (PAD) associated with DM in the context of increased AGE protein. We hypothesize that increased AGE protein expressions in DM PAD is associated with increased neovascularization and inflammation that may enhance plaque progression in DM.
Methods: Twenty two plaques from symptomatic patients collected during superficial femoral artery directional atherectomy were studied. Plaque specimens were grouped as DM (n=11) and no DM (n=11). Immunohistochemistry was performed to quantify AGE protein density with microscopic planimetry. Double label immunochemistry was done to quantify neovascularization and inflammation as previously reported. H& E section was used to grade the medial calcification.
Results: Presented in the Table. The AGE protein density was significantly increased in DM group when compared to no DM group (see Figure). Neovascularization, inflammation, and medial calcification were also increased in DM group (Table). Regression analysis identified correlation between neovascularization and inflammation with AGE protein density (r= 0.56; p=0.03).
Conclusions: Increased AGE product receptors are associated with plaque neovascularization and inflammation in PAD from patients with DM. Early detection of AGE and response to treatment may serve as a marker for disease progression in these patients.
- © 2013 by American Heart Association, Inc.