Abstract 13830: Association of Serum 25-hydroxyvitamin D3 And D2 With Endothelial Function and Arterial Stiffness in Coronary Artery Disease
Background: Vitamin D deficiency is highly prevalent worldwide and is associated with the presence of coronary artery disease (CAD). There are two major forms, vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Endothelial function and arterial stiffness are key players in the pathophysiology of atherosclerotic disease. We study the effect of the different vitamin D fractions (D3/D2) on arterial wall properties in CAD patients.
Methods: We included 252 (mean aged 62±11 years) patients with stable CAD, one month after percutaneous coronary intervention. Endothelial function was evaluated by flow mediated dilation (FMD). Carotid femoral pulse wave velocity (PWV) was measured as an index of arterial stiffness and augmentation index (AI) as a measure of reflected waves. Measures for 25(OH)D2 and 25(OH)D3 were performed using Liquid Chromatography Mass Spectrometry technology. Subjects with vitamin D levels below 20, between 20 to 30 and above 30ng/ml were characterized as having deficiency, insufficiency and sufficiency respectively.
Results: From the study population, 155(62%), 66(26%) and 31(12%) were categorized as having vitamin D deficiency, insufficiency and sufficiency respectively. Subjects with vitamin D deficiency and/or insufficiency had significantly higher D2 to D ratio compared to subjects with vitamin D sufficiency [0.029(0.007-0.039) vs. 0.013(0.012-0.021) vs. 0.009(0.008-0.012), p<0.001]. There was no difference between subjects with vitamin D deficiency, insufficiency and sufficiency in FMD (5.03±2.09% vs. 4.52±2.06% vs. 4.80±2.05%, p=0.31), AI (23.68±8.42% vs. 24.14±9.00% vs. 22.52±11.31%, p=0.71) and PWV (8.94±2.05m/sec vs. 9.26±2.64m/sec vs. 8.42±1.85m/sec, p=0.23). Interestingly, FMD was positively associated with D2 to D ratio (rho=0.13, p=0.02) and subjects with D2 levels<0.3ng/ml had impaired FMD compared to those with increased D2 levels (p=0.048).
Conclusion: Vitamin D insufficiency/deficiency is highly prevalent in CAD subjects. Although vitamin D status is not associated with arterial stiffness and endothelial function, D2 concentrations are positively associated with endothelial function. These findings may suggest a beneficial role of vitamin D2 levels in vascular health.
- © 2013 by American Heart Association, Inc.