Abstract 13827: Cxcr7 Gene Transfer Contributes to Reendothelialization Capacity of Early Endothelial Progenitor Cells From Patients With Coronary Artery Disease
Background: Dysfunction of early endothelial progenitor cells (EPC) is currently considered as a critical factor for the pathogenesis of coronary artery disease (CAD). CXCR7 is recently found as a novel chemokine receptor for SDF-1. However, little is known about the association between CXCR7 signaling and EPC-targeted repair for vascular endothelium following arterial injury in CAD patients. In this study, we investigated the role of CXC chemokine receptor 7 (CXCR7) in the dysfunction of early EPCs from patients with CAD.
Methods: The expression of CXCR7 was measured in early EPCs isolated from 20 CAD patients and 20 healthy volunteers. EPCs from CAD patients were then transduced with adenovirus serotype 5 encoding human CXCR7 gene (Ad5/CXCR7). EPCs function was examined in vitro migration, adhesion and in vivo reendothelialization.
Results: Compared with healthy volunteers, EPCs from CAD patients displayed reduced level of CXCR7 as well as impaired adhesive, migratory capacity in vitro and reendothelialization in vivo after transplantation into nude mice with carotid artery denudation injury. Up-regulation of CXCR7 expression by gene transfer significantly restores the capabilities of EPCs from CAD patients and these functional augmentations ultimately lead to the improvement of EPCs mediated in vivo reendothelialization.
Conclusions: The present study demonstrates that reduced CXCR7 expression contributes to the dysfunction of early EPCs from CAD patients. CXCR7 gene transfer may be a novel therapeutic strategy for the improvement of early EPCs function in patients with CAD.
- © 2013 by American Heart Association, Inc.