Abstract 13758: Tie2, Angiogenin, and Vegf in a Cohort of Patient With Stable Peripheral Artery Disease and Varying Glucose Metabolism
Objective: Recently, stem cell growth factors have gained attention in patients suffering from complications of diabetes. In this study we investigated the angiogenetic proteins: angiogenin (ANG), TIE2, and VEGF in patients suffering from peripheral artery disease and varying glucose metabolism.
Methods: The study cohort of 294 (93 female) patients suffering from peripheral artery disease (Fontaine stage I or II) consisting of 84 normal glucose tolerance (NGT), 80 impaired glucose tolerance (PRE), and 130 with overt type 2 diabetes mellitus (T2DM) of were enrolled. Angiogenin, TIE2, and VEGF were obtained by commercially available ELISA. Statistical analyses were done with students’ unpaired t-test, correlation analysis and multivariate regression modeling as appropriate.
Results: Patients with PAD and overt T2DM had significantly increased TIE2 levels compared to patients with NGT (33 (26;40) vs. 28 (24;36) ng/mL, p=0.003), but not increased ANG (541 (378;695) vs. 468 (352;706) ng/mL, p=0.188) or VEGF (553 (384;976) vs. 527 (314;758) pg/mL, p=0.287) levels. Although TIE2 and ANG levels correlated significantly in the study cohort (p<0.001, R=0.222) subgroup analysis revealed a significant correlation of TIE2 and ANG in only patients with NGT (p=0.041, R=0.269), but not PRE (p=0.065, R=0.211) or T2DM (p=0.053, R=0.172). VEGF showed no significant connection to TIE2 (p=0.654, R=0.046) or ANG (p=0.117, R=-0.162). Additionally, TIE2 (33 (26;38) vs. 25 (20;30) ng/mL, p<0.001) and ANG (523 (391;706) vs. 434 (288;520), p=0.012) levels decreased in patients suffering from systemic atherosclerosis. In multivariate regression modeling TIE2 was independently associated with diabetes status (Beta=0.183, p=0.002), atherosclerotic burden (Beta=0.176, p=0.001) and ANG (Beta=-0.195, p=0.001).
Conclusion: We are first to report an increase of TIE2 in patients with type 2 diabetes mellitus, but not ANG or VEGF in our cohort of PAD patients. Additionally, TIE2 levels were diminished in patients with NGT to PRE in systemic atherosclerosis. Contrary, in patients suffering from type 2 diabetes mellitus TIE2 levels were unaffected by systemic atherosclerosis, indicating a possible increase of the angiogenetic TIE2 in those patients.
- © 2013 by American Heart Association, Inc.