Abstract 13757: The Transcoronary Gradient of Circulating Mir-34a is Associated With the Development and Progression of Coronary Atherosclerosis - Insights From Oct-studies
Introduction: Micro-RNAs were experimentally shown to contribute to atherosclerotic plaque formation and stability. In order to establish a potential pathophysiological role of miR-34a,a regulator of endothelial senescence and apoptosis,we correlated transcoronary concentration-gradients of miR-34a with coronary pathomorphology.
Methods: Blood samples were simultaneously obtained from the aorta and the coronary sinus in 35 patients and plasma concentrations of miR-34a were measured by PCR. Comprehensive evaluation of the epicardial artery vessel wall by optical coherence tomography (OCT) was performed to quantitate coronary plaque load, the extent of coronary remodelling as well as the quality of coronary plaques along the entire vessel length of the LAD and LCX.
Results: There was a significant positive correlation for transcoronary gradients of miR-34a with the overall coronary plaque burden (r=0.6; p<0.01). Importantly, a high percentage of stable coronary atheromas did not show an association with the transcoronary gradient of miR-34a (r=0.09; p=0.97), whereas an increasing percentage of unstable thin-cap fibroatheromas was associated with a significant release (r=0.42; p<0.01) of miR-34a into the coronary circulation. Likewise, the extent of coronary remodelling was negatively correlated (r=-0.43; p=0.02) with transcoronary miR-34a gradients with a selective (p<0.01) release of miR-34a during transcoronary passage in the case of predominant negative vessel remodelling. Calculating C-statistics revealed that the transconary gradients of miR-34a, but not of the established biomarkers hs-CRP (C=0.34), hs-Troponin (C=0.6) and NT-pro-BNP (C=0.44) provided excellent diagnostic information of miR-34a for the detection of a high percentage of vulnerable plaques (C= 0.80).
Conclusions: The present study is the first to document an association between vulnerable coronary plaque load and the release of the athero-aggressive miR-34a. The results demonstrate a close association between increasing coronary plaque load, plaque instability and vessel-remodelling and transcoronary gradients of miR-34a , and indicate the potential of measuring miR-34a as a useful biomarker for the identification of vulnerable patients.
- © 2013 by American Heart Association, Inc.