Abstract 13742: In Body Tissue-engineered Heart Valve (Biovalve) Architecture Based on 3D Printer Molding
Purpose: In body tissue architecture technology, attempted as novel and practical regeneration medicine by us, can prepare a completely autologous heart valve, called Biovalve, consisting of only recipient’s own tissue according to the shape of mold faithfully. In this study, 3D printer was used for design of the preparation molds. Four different types of heart valves with or without stent impregnation were individually created with intended size in several animal models.
Methods and Results: 3D printers (Projet or Objet) could realize easily the 3D-shape and size of native heart valves regardless of types within several hours. Only 1-month subcutaneous embedding of the assembling of 2 conduit parts and 3 sinus parts produced aortic or pulmonary valve-shaped Biovalves from completely autologous connective tissue with collagen and fibroblasts. Upon implantation of the Biovalves in a goat model good valvular function (regurgitation: less than 15%) was obtained with high durability (over 1 month) at mean flow rate of 4.8 L/min, AoP of 129-49 (82.2) mmHg, and beating rate of 70. Combination with stents (Goodman Co.) at the mold embedding formed stent-impregnated Biovalves. By catheter-induced implantation of the Biovalves TAVI in a goat model or TPVI in a canine model were performed. In addition, mitral and tricuspid valves were similarly formed by 3D molding in body. Their leaflets and tendinous cords were connected robustly and seamlessly. In a canine model, after surgical replacement postoperative echocardiography showed smooth movement of the leaflets with little regurgitation under systemic circulation. In all implantation study, the luminal surface after implantation was very smooth and fully covered with thin neointima including endothelial cells without thrombus formation.
Conclusion: Functional, autologous, 3D-shaped, aortic, pulmonary, mitral, and tricuspid valves with clinical application potential were formed by only in body embedding of specially designed molds, which could be prepared by 3D printer within several hours.
- © 2013 by American Heart Association, Inc.