Abstract 13653: Branched-Chain Amino Acids Ameliorate Heart Failure in Rats
Introduction: Heart failure (HF) is associated with changes in energy metabolism of the heart, as well as in extra-cardiac organs such as skeletal muscles. Particularly, abnormal mitochondria are observed in both heart and skeletal muscles. Branched-chain amino acids (BCAA) reportedly promote mitochondrial biogenesis in heart and skeletal muscles.
Hypothesis: We assessed the hypothesis that BCAA ameliorate HF.
Methods: Dahl salt-sensitive (DS) rats fed a high-salt (HS) diet from 6 weeks of age were used as a model of HF. BCAA (1.5 mg/g body weight/day) were administered in drinking water from 11 weeks of age. DS rats fed a low-salt (LS) diet and tap water as a control (LS-C, n=8), DS rats fed a LS diet and BCAA (LS-BCAA, n=8), DS rats fed a HS diet and tap water (HS-C, n=30), and DS rats fed a HS diet and BCAA (HS-BCAA, n=30) were used. Survival and cardiac function were monitored, and animals were sacrificed at 21 weeks of age and analyzed.
Results: BCAA improved the survival of rats with HF (HS-BCAA 57% and HS-C 37%, p=0.030). BCAA preserved the cardiac systolic function as assessed by fractional shortening (HS-BCAA 45±3% and HS-C 35±4%, p=0.033). The body weight was decreased in HS-C compared with LS-C (HS-C 364±11 g and LS-C 448±6 g, p<0.001), and BCAA inhibited the body weight loss (HS-BCAA 393±6 g and HS-C 364±11 g, p=0.037). Proliferator-activated receptor-γ coactivator (PGC)-1α was decreased in heart tissue of HS-C compared with that of LS-C. PGC-1α expression was not changed in the heart of HS-BCAA compared with that of HS-C, but it was increased in the skeletal muscles of HS-BCAA compared with that of HS-C, associated with the increased expression of genes involved in mitochondrial respiration.
Conclusion: BCAA prolonged survival and preserved the body weight and the cardiac function in a rat model of HF, associated with the increased expression of genes involved in mitochondrial biogenesis and function in skeletal muscles.
- Heart failure
- Cardiac metabolism
- Mitochondrial energetics, heart failure, arrhythmias
- © 2013 by American Heart Association, Inc.