Abstract 13540: Inhalation of H2 in the Absence of Hyperoxia Starting after Rosc Improves Neurologic Outcomes and Survival in a Rat Model Following Resuscitation from Cardiac Arrest
Objective: Hydrogen (H2) gas has potential as a novel antioxidant. We have previously described that inhalation of 2% H2 gas in the presence of hyperoxia (98% O2) starting at the beginning of CPR and given for 2 hours after return of spontaneous circulation (ROSC) significantly improves the functional status of the brain and heart in a rat model. We investigated whether inhalation of H2 in the absence of hyperoxia starting after ROSC improves neurologic outcomes in a rat model following resuscitation from cardiac arrest.
Methods&Results: After 6 min of untreated cardiac arrest by ventricular fibrillation, rats received manual chest compression and ventilated with 100% oxygen. After 3 minutes of cardiopulmonary resuscitation, animals received a defibrillation shock of 2J. At 5 minutes after ROSC, animals were randomized into 4 groups: ventilated with 26% O2 under normothermia (37°C) (control), 26% O2 plus 1.3% H2 under normothermia (H2), 26% O2 under hypothermia (33°C) (TH), or 26% O2 plus 1.3% H2 under hypothermia (H2+TH). Gas inhalation continued for 2 hours and body temperature was controlled for 4 hour after ROSC. The survival rate 7 days after ROSC was significantly improved in H2 (66.7%), TH (66.7%), and H2+TH (83.3%) compared to control (33.3%). Both H2 and TH equally improved neurologic deficit score and attenuated loss of CA1 pyramidal neurons and microtubule-associated protein 2 (MAP2) expression in the hippocampus at 7days after ROSC. The combination of the two (H2+TH) further improved such delayed hippocampal neuronal injury.
Conclusion: Inhalation of H2 in the absence of hyperoxia starting after ROSC improves neurologic outcomes and survival to the same levels as TH in a rat model following resuscitation from cardiac arrest. Combined therapy with H2 inhalation and TH had an additive effect.
- Post cardiac arrest care
- Cardiopulmonary resuscitation
- Ischemia reperfusion
- Oxidative stress
- Post cardiac resuscitation
- © 2013 by American Heart Association, Inc.