Abstract 12910: Feasibility Of Connective Tissue Membrane (Bio-Sheet) Prepared In The Body As Cardiovascular Repair Material As A Substitute For Self-pericardium
Background: Autologous pericardium is commonly used for the reconstruction of intracardiac structures and pulmonary artery, although the amount of pericardium that can be harvested from one patient is limited. To overcome this issue, tissue engineered material that has potential to grow and assimilate to native tissue is desired.
We developed autologous prosthetic tissues using “in-body tissue architecture” technology, which is a novel and practical approach of regenerative medicine based on the tissue encapsulation phenomenon of foreign materials in living bodies. We evaluated the durability and safety of connective tissue membrane (Bio-Sheet) used as an atrial wall patch.
Materials and Method: Six square acryl chips (size: 20х20 mm, 2 mm in thickness) were implanted into subcutaneous pouches of beagles. After 8 weeks, we harvested the chips covered with connective tissue, and we obtained the connective tissue membrane (Bio-Sheet) by removing the chip. Bio-Sheet had almost equal strength compared to autologous pericardium.
Under general anesthesia and right lateral thoracotomy, we made a right atrial defect (size: 20 mmх10 mm) by using side-binding aortic clamps and closed the defect with Bio-Sheet patch (Fig. 1A, n=6). We did not use any antiplatelets and anticoagulant after the implantation.
Results: We evaluated the implanted beagle dogs 7 days (n=2), 30 days (n=2), and 3 months (n=2) after the patch repair. No aneurysmal change was found in the Bio-Sheet patch macroscopically (Fig. 1B showing view from intraatrial side). In histopathological evaluation, the surface of the atrial patch was completely covered by endothelial tissue and neointima, without formation of intraatrial thrombosis 3 months after the patch closure.
Conclusion: Bio-Sheet, which can be made and implanted easily, showing a fine mid-term result, may have potential to be used as a substitute for self-pericardium in cardiac surgery including congenital cardiac surgery.
- © 2013 by American Heart Association, Inc.