Abstract 12179: Evaluation of 3D Vasa Vasorum Volume Using Coronary Optical Coherence Tomography in Transplant Patients
Backgrounds: Vasa vasorum (VV) is a network of microvessels located in the walls of arteries and veins, and responsible for progression of coronary lesions in both native atherosclerosis and in cardiac allograft vasculopathy (CAV). Although we have recently reported the efficacy of micro-computerized tomography to assess VV in vitro, the methods to evaluate VV in vivo remain to be established. The purpose of this study is to examine adventitial VV volume of human coronary artery with 3D images reconstructed from optical coherence tomography (OCT) in transplant patients.
Methods and Results: We enrolled 5 transplant patients (Men/Women 4/1, Age range 24 - 66 years old, one year after heart transplantation) referred to coronary angiography and OCT for evaluation of CAV, from September, 2011 to June, 2012. OCT imaging was performed in left anterior descending artery, and recorded over 50 mm divided into 5 ten-mm-segments of 50 slices each. Adventitial VV were identified as low intensity areas with major diameters between 50-300 micrometer, which were located within 1 mm from the lumen-intima border. VV areas and the boundaries of lumen-intima and media-adventitial were traced manually in all slices. Subsequently, 3D images were reconstructed using the ANALYZE software 11.0 (Biomedical Imaging Resource, Rochester, Minnesota). In each segment, the volumes of the VV, lumen, and vessel were calculated. The plaque volume was calculated by subtracting lumen volume from vessel volume. Figure A shows representative 3D image and the segmental VV volume and PV. Median values [25%, 75% quartiles] of VV volume and PV were 1.22 mm3 [0.76, 1.58] and 19.4 mm3 [15.4, 22.9], respectively. There was significant correlation between VV volume and PV (r2 = 0.74, P ≤ 0.001, Figure B).
Conclusion: Our results suggest 3D reconstruction of adventitial VV from OCT images as a novel method to estimate VV volume in vivo in transplant patients and further support the role for the VV in CAV.
- © 2013 by American Heart Association, Inc.