Abstract 10995: Effects of Renal Sympathetic Denervation on Cardiac And Renal Function in Diabetic Ren-2 Rats With Diastolic Heart Failure
Back ground Diabetic cardiomyopathy (DCM) is characterised by sympathetic over-activity, and associated with hypertension, sodium retention and hyperkalemia. We determined the renal and cardiac functional effects of renal sympathetic denervation (RDN) in an animal model of DCM.
Methods: Six week old streptozotocin-induced diabetic homozygous (mRen-2)27 transgenic rats were randomised to receive sham operation (n=6) or RDN (n=6) and followed for 6 weeks. RDN was performed surgically by stripping the adventitia of renal arteries and veins, dissecting around sympathetic nerve bundles and coating them with a solution of 10% phenol in ethanol. Prior to sacrifice, urine sodium and potassium were obtained from 24 hour metabolic caging and animals underwent echocardiography and pressure-volume loop analysis. Blood pressure (BP) was measured in conscious rats using the tail-cuff method on the day before the sacrifice. Cardiac catheterization was performed under pentobarbitone anaesthesia. SVR was calculated as mean BP/CO, arterial stiffness as PP/SV and left ventricular wall tension (LVWT) as ejection systolic pressure x LV wall thickness.
Results: In RDN rats, BP, LV end-systolic pressure and LV dimensions were significantly reduced compared to sham. Arterial stiffness and LVWT showed a trend toward reduction by RDN. There were no significant differences in glomerular filtration rate, cardiac output or HR in RDN rats. Renal sodium excretion was border line increased; urinary potassium excretion was significantly increased by RDN.
Conclusions: RDN results in reduced BP whist and LV with an increase excretion in urinary sodium and potassium excretion. These findings suggest potentially important kaliuretic and natriuretic effect of RDN associated with improved cardiac hemodynamics in diabetic heart failure.
- © 2013 by American Heart Association, Inc.