Abstract 10888: Increased MicroRNA-126 And MicroRNA-199 Expression In Circulating Microparticles Is Associated With Reduced Risk For Cardiovascular Events
Background: Circulating microRNAs (miRs) are differentially regulated and selectively packaged in microvesicles in patients with coronary artery disease (CAD). We evaluated whether circulating miRs in patients with stable CAD have prognostic impact on the occurrence of cardiovascular events.
Methods and Results: 10 endothelial-expressed miRs including miR-126, miR-222, miR-let 7d, miR-21, miR-20a, miR-27a, miR-92a, miR-17, miR-130 and miR-199a in plasma and circulating microparticles (MPs) were quantified by real-time polymerase chain reactions in 181 stable CAD patients. The median follow-up time for major adverse cardiovascular event (MACE)-free survival was 6.1 (6.0/6.4) years. Five patients were lost to follow-up. A first MACE occurred in 55 (31.3%) patients. There was no significant association between cardiovascular events and plasma levels of these 10 endothelial-expressed miRs. In contrast, an increased expression of miR-126 and miR-199a in circulating MPs (MmiR) was significantly associated with a reduced MACE rate. In multivariate analysis, higher level of MmiR-126 was identified as an independent predictor of survival free of MACE (hazard ratio: 0.381, 95% CI: 0.190-0.764; P=0.007) and revascularization (hazard ratio: 0.391, 95% CI: 0.178-0.861; P=0.02). Likewise, and increased level of MmiR-199a was associated with a reduced risk of MACE (hazard ratio: 0.414, 95% CI: 0.211-0.812; P=0.01) and revascularization (hazard ratio: 0.305, 95% CI: 0.135-0.691; P=0.004) in multivariate analysis.
Conclusion: The level of MmiR-126 and MmiR-199a predict the occurrence of cardiovascular events and may help to identify patients with CAD at increased cardiovascular risk.
- © 2013 by American Heart Association, Inc.