Abstract 10678: Increase in the Number of Circulating Primordial Cells is Associated With Improved Left Ventricular Function in Dilated Cardiomyopathy
Introduction: Primordial cells are unipotent cells committed to a germ lineage. In a prior study, we observed that increased numbers of circulating primordial cells induced by statin therapy were associated with improvement in left ventricular ejection function (LVEF).
Hypothesis: We tested the hypothesis that increased numbers of circulating primordial cells are directly related to LVEF in patients with dilated cardiomyopathy.
Methods: Our patient sample consisted of patients seen in the heart failure (CHF) clinic with an LVEF < 30% at the time of initial CHF diagnosis. Two patient groups were identified: those with > 10% increase in LVEF with standard medical therapy (group A), and those with < 10% increase in LVEF with standard medical therapy (group B). Patients with inflammatory disease or medications known to alter the number of circulating cells were excluded. Blood samples were obtained and circulating primordial cells were detected using the commercially available Aldofluor kit assay label for stem and progenitor cells. Cells were quantified by flow cytometry and expressed as percentage of all detected cells. Linear regression was done to evaluate the relationship between the number of circulating primordial cells and LVEF.
Results: Twenty-eight patients (14 in each group) were enrolled. Mean age was 57 ± 11 years. Nineteen (68%) were male. The mean ± standard deviation of LVEF at the time of blood acquisition was 44 ± 11% in group A and 26 ± 9% in group B patients (p= 0.0001). There was a significant trend (p=0.1) towards a direct relation between LVEF and number of circulating primordial cells with a correlation coefficient of 4.30.
Conclusion: In conclusion, our study shows that an increase in the circulating number of primordial cells is associated with an increase in LVEF. This trend to the positive relation represents a strong signal relating ventricular performance to circulating numbers of primordial cells and suggests that a repair function of circulating pluripotential cells contributes to recovery of LVEF. Our data suggests that strategies to increase both bone marrow production and release of these primordial cells are a promising strategy to treat systolic heart failure patients in the future.
- © 2013 by American Heart Association, Inc.