Abstract 10385: A Newly Developed Technique of Synchrotron Radiation Coronary Micro-angiography in an In-vivo Rat
Background: The conventional angiography can visualize the 300 um diameter coronary artery, but not the proliferations of collateral arteries in ischemic heart disease and of new blood vessels in regenerative medicine that occur at smaller arteriole size. We have developed a system of synchrotron radiation coronary micro-angiography (SRCA) and reported that the minimum identi[[Unable to Display Character: ﬁ]]ed arteriole diameter was 50 um in the Langendorff perfused rat heart. However, in-vivo rat SRCA has the problem that temporal resolution is compromised because of the high heart rate in rats. Recently, SRCA using a high-speed rotating shutter system has been reported. In this study, we aimed to perform SRCA in an in-vivo rat using the bradycardic agent method.
Method: SRCA was performed at the Photon Factory of the High Energy Accelerator Research Organization (Tsukuba, Japan). The advantages of synchrotron radiation derived X-rays are high spatial resolution due to increased photon density and straightness of beam. High density resolution is obtained with the high-gain avalanche rushing amorphous photoconductor camera tube using a fiber-optic plate. Rats were anesthetized. The micro polyethylene tube for angiography was inserted into the carotid artery. SRPA was performed after the bradycardic agent was given intravenously.
Result: High spatial and density resolution images were obtained. The minimum identified coronary artery diameter was 100 um in an in-vivo rat. The special feature of our images is that they are motion pictures, not still images. The still image shown below is captured from our video.
Conclusion: These results demonstrate the effectiveness of SRCA for visualizing the coronary artery in an in-vivo rat. Our method of using a bradycardic agent was simple and could improve the temporal resolution of SRCA. This newly developed technology may help investigate the proliferations of collateral arteries in ischemic heart disease and of new blood vessels in regenerative medicine.
- © 2013 by American Heart Association, Inc.