Abstract P282: Effects of a Single Unit of Different Alcoholic Beverages on Urinary Excretion Profiles
Background: Excessive alcohol consumption has been shown to cause harmful effects such as cancer, diabetes, assault and accidents. Moderate alcohol consumption, especially of red wine, has been suggested to be beneficial for the prevention of heart disease.
Objective: The aim of this study is to use proton nuclear magnetic resonance (1H NMR) based metabolic profiling approach to evaluate the metabolism of 5 different alcoholic beverages. We also aim to identify potential urinary metabolites that are related to the intake of different alcohol beverages.
Methods: 14 men and 15 women, aged 21 - 50, each was given one unit (8 gram of alcohol) of different alcoholic beverages (Stella Artois or Foster’s lager, gin, red or white wine). All participants provided eight urine specimens, at baseline (immediately before alcohol consumption), and every 2 hrs thereafter until 12 hrs post alcohol ingestion and again after 24 hr post intake of alcohol. Spectra were acquired using a standard one dimensional experiment. Principle component analysis and pairwise orthogonal partial least squares-discriminant analysis (OPLS-DA) comparison were made firstly, between baseline and at each time point; and secondly, between different types of alcohol intake at different time points. The area under the curve for ethanol peak (at δ 1.18) and ethyl glucuronide peak (at δ 1.24) were calculated and compared between different groups.
Results: Preliminary analysis of the OPLS-DA comparison between baseline and at each time point after alcohol consumption, ethanol and ethyl glucuronide were the key metabolites differentiating baseline urinary spectral data from those post alcohol ingestion. In addition to alcohol derived metabolites, several endogenous metabolites (e.g., hippurate, histidine, and 1-methylhistidine) were also found to be either positively or negatively correlated to alcohol consumption. In general, the ethanol peak was not visible at 6 hrs while ethyl glucuronide was visible until 8 hrs post ingestion. Red wine drinkers generally had a lower urinary ethanol level compared to those who drank Foster’s, Stella, gin or white wine. Whilst urinary ethyl glucuronide levels were higher in Foster’s/Stella drinkers compared to other drinkers up to 6 hrs post ingestion.
Conclusion: Preliminary results showed that the rate of excretion of alcohol and its related metabolites differ between different types of alcoholic beverage. This may aid our understanding on the exposure and metabolism of different alcohol beverages, and may aid in our understanding of secondary metabolic effect of alcohol consumption. 1H NMR analysis of urine specimens may also be useful to identify potential new alcohol metabolites but this would need to be further validated in larger human studies.
- © 2013 by American Heart Association, Inc.