Abstract P182: Mobile Source Air Pollution is Associated with the Plasma Concentration of Multiple Acylcarnitines in a Large Cardiovascular Cohort
CATHGEN is a large (N = 9334) sequentially-sampled cohort of patients presenting to the Duke University Cardiac Catheterization Laboratory from 2001-2011. With residential location for 8079 (86.5%) of cohort individuals, we have created a powerful resource to understand the relation between environmental exposures and cardiovascular disease (CVD).
In this study we used 843 CATHGEN participants in the tri-county area of Durham, Wake, and Orange counties, NC (DWO) to identify associations between mobile source air pollution and 61 specific metabolites from a targeted metabolic profiling panel. We used distance to the nearest primary or secondary roadway (DTR) as a proxy for mobile source air pollution and adjusted each association for race, sex, smoking status, and socioeconomic status. Metabolite concentrations were log-transformed before analysis.
In DWO we saw multiple associations between DTR and both short and long-chain acylcarnitines. DTR was associated with an increase in the short-chain acylcarnitines C5:1 (β = 0.087 P = 0.033) and C5-OH/C3-DC (β = 0.777, P = 0.016). Plasma concentrations of acylcarnitine C18:2 were lower among study participants living closer to roadways (β = -0.056, P = 0.008). We saw modest associations between DTR and acylcarnitines C14-OH/C12-DC (β = 0.250, P = 0.063), C18:1 (β = -0.036, P = 0.051), and C18-OH/C16-DC (β = 0.645, P = 0.056).
C18:2, C14-OH/C12-DC, C18:1, and C18-OH/C16-DC are all related to β-oxidation. We saw negative associations with distance to roadways for C18:2 and C18:1 acylcarnitines and positive associations with DTR for C14-OH/C12-DC and C18-OH/C16-DC. β-oxidation is an important source of energy in the mitochondria and altered concentrations of β-oxidation related acylcarnitines are often markers of mitochondrial dysfunction. Further studies are needed to explore the relationship between β-oxidation related acylcarnitines and mobile source air pollution.
Our two remaining associations were C5:1 and C5-OH/C3-DC. C5:1 is related to branched chain amino acid metabolism. In published analyses branched chain amino acids were associated with coronary artery disease (CAD) prevalence, with higher plasma concentrations of branched chain amino acids seen in CAD cases as compared to controls.
In conclusion we examined a targeted metabolic panel of 61 metabolites for association with distance to roadways, a proxy for mobile source air pollution. We saw associations between specific long-chain β-oxidation related acylcarnitines as well as with two short-chain acylcarnitines, one related to branched chain amino acid metabolism. Using the clinical and molecular data available in CATHGEN we will explore these associations further to determine if specific genes and pathways related to these acylcarnitines are also associated with mobile source air pollution.
- © 2013 by American Heart Association, Inc.