Abstract P100: Plasma Phospholipid Fatty Acid Composition as a Biomarker of Habitual Dietary Fat Intake: The INTERMAP/INTERLIPID Study
INTRODUCTION An inverse association between dietary intake of marine-derived n-3 fatty acids and risk of cardiovascular diseases has been described. Accurate assessment of fat intake is essential to examine relationships between diet and disease risk but estimating individual intakes of fat quality by dietary assessment is difficult. To address this problem, we assessed association of plasma phospholipid (PL) fatty acid levels with dietary fatty acid intakes in the INTERMAP/INTERLIPID Study, conducted with a standardized protocol. Participants were Japanese in Japan and Japanese-Americans in Hawaii.
METHODS Plasma PL fatty acids and nutrient intakes were examined by standardized methods in men and women ages 40-59 years from five population samples, one from Hawaii (100 men, 106 women), the other four from Japan (565 men, 566 women). Habitual fatty acid intakes were estimated from four standardized 24-h dietary recalls conducted with each participant at four visits by certified dietary interviewers. Plasma PL fatty acid composition was measured by gas chromatography in a central laboratory. Correlation coefficients were calculated between plasma PL fatty acid levels and fatty acid intakes.
RESULTS Mean fatty acid intakes were: palmitic acid 9.9 g/day (4.3%kcal), oleic acid 20.0 g/day (8.6%kcal), alpha-linolenic acid 1.8 g/day (0.8%kcal), eicosapentaenoic acid (EPA) 0.3 g/day (0.1%kcal), docosahexaenoic acid (DHA) 0.6 g/day (0.3%kcal), and linoleic acid 12.2 g/day (5.3%kcal). Mean plasma PL fatty acid composition was: palmitic acid 27.8%, oleic acid 8.3%, alpha-linolenic acid 0.2%, EPA 3.6%, DHA 8.0%, and linoleic acid 21.5%. Pearson correlations between dietary fatty acid (%kcal) and plasma PL fatty acid composition (%) values were: palmitic acid -0.09, oleic acid -0.04, alpha-linolenic acid 0.17, EPA 0.63, DHA 0.48, and linoleic acid 0.42.
CONCLUSIONS We observed high correlations between dietary fatty acid intakes and plasma phospholipid fatty acids for marine-derived n-3 fatty acids and for linoleic acid -- but not for other fatty acids -- in participants from Japanese and Japanese-American populations with large variations in dietary fatty acid intakes. Plasma phospholipid fatty acid measurement has limited utility for assessment of dietary fatty acid intake.
- © 2013 by American Heart Association, Inc.