Abstract 6: Bone Marrow Mesenchymal Stem Cells Improved Survival in Septic Rats Induced by Cecal Ligation and Puncture
Background: Some studies have suggested that bone marrow mesenchymal stem cells (BMSCs) have beneficial effects on damaged nerves, ischemia/reperfusion injuries and so on. However, their effects on septic shock have never been scrutinized. In this study, we evaluated effects of BMSCs on septic rats
Methods: Forty male Sprague-Dawley rats were randomly assigned to either of the following groups: group C (n=20), no medication following cecal ligation and puncture (CLP); group M (n=20), BMSCs were administered following CLP. BMSCs were collected from right and left femurs of two rats, using a specially designed devise for that purpose and administered to one rat in either group. Rats in both groups were anesthetized with intraperitoneal pentobarbital and then underwent cecal ligation and puncture. The puncture was performed with 18G needle and 2 holes were made to disseminate inflammation. After skin closure, rats in group M were given BMSCs intravenously. All the rats were observed for the following 7 days. Mortality was calculated for the 7-day observation period.
Result: The mortality rates at 7 days in group M and group C were 30 % and 65%, respectively. There was a significant difference in mortality rate between group M and C. (p<0.05)
Conclusions: Bone marrow mesenchymal stem cells improved mortality in septic rats induced by CLP. This result shows us the potential effect of BMSCs to alleviate systematic inflammation that could result in multiple organ failure. Further studies are underway to clarify the underlying mechanism.
- © 2012 by American Heart Association, Inc.