Abstract 18007: Assessment of Pulmonary Artery Morphology by Optical Coherence Tomography:comparison with Histology
Background: Since its introduction, optical coherence tomography (OCT) has been primarily used for the diagnosis of coronary artery disease. A few feasibility studies of OCT to visualize the pulmonary arteries were reported. However, OCT findings in the pulmonary arteries have not been validated using histology as the gold standard.
Methods: To validate OCT findings for pulmonary arterial imaging, we selected 27 pulmonary arteries from 11 cadavers. Comparison of OCT images and histology was performed.
Results: The wall of the normal pulmonary artery was a single-layered structure with homogeneous signal-rich bands. An interface between the lumen and the wall was clearly visible, however, the outer border of the wall was less well demarcated. Vasa vasorum was visualized surrounded by lacunae of different shapes (alveoli). The vessel diameter (mean = 2.136 ± 0.331 mm) and wall thickness (mean = 0.162 ± 0.027 mm) were measured by OCT. The Pearson correlation showed that the pulmonary arterial wall thickness measured by OCT and histology had an excellent correlation (r = 0.837, P < 0.0001). Bland-Altman analysis showed a mean difference of 0.002 mm, and 1.96 SDs of the mean were 0.026 mm and -0.031 mm. Thus, pulmonary arterial wall thickness as measured by OCT imaging was comparable to that measured by histology.
Conclusion: The pulmonary arterial wall has a single-layered structure with an average thickness of 0.162 mm. There was a strong correlation between histology and OCT measurements of the pulmonary arterial wall thickness.
Keywords: pulmonary artery, optical coherence tomography, histology, imaging Figure: Normal pulmonary artery image. Vasa Vasorum (single arrow), alveoli tissues (double arrows). A: OCT image show a single-layered structure with homogeneous signal-rich bands. B: Corrresponding histology (original magnification, ×40).
- © 2012 by American Heart Association, Inc.