Abstract 17210: Neuroglobin Overexpression Protects Against Myocardial Ischemia by Attenuation of Hypoxia Inducible Factor-1alpha Related Inflammatory Pathways
Introduction: Neuroglobin (Nbg) is an oxygen-binding globin mainly expressed in nervous tissues. Ngb overexpression has been shown to have cytoprotective effects during brain ischemia. We investigated the effects of Ngb overexpression on survival, infarct size and hypoxia-induced gene expression in a mouse model of acute myocardial infarction (AMI).
Methods & Results: AMI was induced by LAD ligation in wild type (WT, n=44) and Ngb overexpressing mice (n=20). Ngb overexpression significantly enhanced survival (p=0.029) and reduced myocardial infarction size (p=0.045) compared to WT. In the infarction border zone, the mRNA expression of HIF-1a (p<0.003) and hypoxia-induced vascular adhesion molecules ICAM1 (p<0.001) and VCAM1 (P<0.002) was significantly attenuated in Ngb overexpressing mice. Unexpectedly, we did not evidence differences in NOS3 mRNA expression in the infarction border zone.
Conclusions: Ngb overexpression enhances survival and reduces infarction size after AMI. Mechanistically, reduction of tissue hypoxia and attenuation of hypoxia-induced inflammatory pathways could be responsible for the beneficial effects of Ngb overexpression in the setting of AMI.
- © 2012 by American Heart Association, Inc.