Abstract 16797: Anisotropic Cardiac Patch Promotes Electromechanical Integration of Transplanted Cells and Therapeutic Efficacy after Infarction
Background− Heart is an extremely sophisticated organ with anisotropic structure, contractility and electro-conductivity; however, rare were there studies addressing the cardiac anisotropy of cell transplantation for myocardial repair. Here, we hypothesize that the graft’s anisotropy of myofiber orientation, contractility, and electro-conductivity would determine the therapeutic outcome of cardiac renovation. Methods and Results− We developed aligned- or random-orientated nanofibrous electrospun patch (aEP and rEP, respectively) which was seeded with cardiomyocytes (CMs) and endothelial cells (ECs) to test the hypothesis. We demonstrated that CMs performed an orderly manner in arrangement, lengthened shape in morphology, enhanced amplitude in beating, and significant synchronization in integrated contraction when cultured on aEP rather than rEP in vitro. We thus further tested the therapeutic efficacy in a rat myocardial infarction (MI) model (n ≥ 10 for each group; Figure). Our results indicated that the aggravation of post-MI injury can be retarded by implantation of EP, which was due to the mechanical supporting. Surprisingly, cardiac deterioration occurred when cells were transplanted along with rEP, due to inadequate integration of graft with the host and defective electro-coupling. Significantly, the functional integration between graft and host and the cardiac performance were improved only when transplanted cells were grafted on aEP at 56 days after MI.
Conclusions− To our knowledge, this is the first study providing in vivo functional characterization and therapeutic efficacy of anisotropic cardiac patch. Importantly, the results demonstrate the significance of cardiac anisotropy recapitulation in myocardial tissue engineering, which should be considered and is valuable for future studies and clinical applications.
- © 2012 by American Heart Association, Inc.