Abstract 15065: Marble-Burying Behavior-Induced Unstable Plaques are Stabilized by Exercise Training in Experimental Atherosclerosis
BACKGROUND: Stress may play a significant role in the development of atherosclerosis.
PURPOSE: We assessed the hypothesis whether behavioral stress may affect the development of atherosclerosis and whether concomitant regular exercise training may influence the composition of atherosclerotic plaques in apolipoprotein (apo) E-deficient mice.
METHODS: Atherosclerosis was induced in apo E-deficient mice fed a high fat diet. Behavioral stress was provoked by glass marble-burying procedure at each time with/without exercise training (45 min swimming, 3 times/week). Mice were treated over 8 weeks (each group, n=10∼16).
RESULTS: The plaques containing macrophage accumulation with intercellular adhesion molecule-1 (ICAM-1) expression associated with reduced collagen contents were induced in the mice treated with marble-burying. However, ICAM-1 expression was suppressed and collagen contents were reversed in the mice that received marble-burying behavior plus exercise training. In addition, concomitant exercise training reduced superoxide production in aortic walls, shown by dihydroethidium staining, compared with that in mice with marble-burying behavior alone. Differences in lesion area did not correlate with any significant alterations in serum lipid levels.
CONCLUSIONS: Behavioral stress induced the unstability of atherosclerotic plaques in apo E-deficient mice. Exercise training may stabilize unstable plaques induced by marble-burying behavior by the suppression of superoxide production in this animal model.
- © 2012 by American Heart Association, Inc.