Abstract 13964: Intramyocardial Injection of Adipose Derived Stem Cells and Endothelial Cells Increased Pericardial Fat After Myocardial Infarction in Rats

Abstract

Background : Intramyocardial injections of mouse adipose derived stem cells (mADSCs) and mouse endothelial cells (mECs) have been known for improving myocardial function after acute myocardial infarction (AMI). However, the effects of injected mADSCs and mECs on pericardial fat and their association with improvement in myocardial function have not been studied.

Methods : Twenty MI rats were divided into 4 groups; Control, mADSCs, mECs and mADSCs+mECs groups, and 5 x 10⁵ cells were injected around three peri-infarct areas after AMI. Echocardiography and peripheral blood cytokines were measured at day 1, 7, 14, and 28 after AMI. Flow cytometry was used to analyze the origin of pericardial cells after isolating with collagenase type I. Also adipokines were measured. Masson’s trichrome(MT) staining was used to measure cross-sectional MI and pericardial fat area at papillary muscle level.

Results : The weight of pericardial fat was significantly higher in the mADSCs group compared to the Control, mECs, and mADSCs+mECs groups (0.168±0.045g, 0.054±0.038g, 0.023±0.021g, 0.11±0.052g, P<0.001, respectively). The increased pericardial fat originated from rat was confirmed by increased anti-rat specific antibodies such as CD90 and CD161 in the pericardial fat (Figure 1). Pericardial fat area was significantly higher in the mADSCs group compared to the other three groups. The increases from baseline in left ventricular ejection fraction and anti-inflammatory cytokine such as IL-10 were significantly higher in the mADSCs group compared to the Control, mECs, and mADSCs+mECs groups (8±4%, 0±3%, 2±3%, 3±3%, P<0.001, respectively), (220±85 pg/mL, 14±14 pg/mL, 45±37 pg/mL, 110±91 pg/mL, P<0.001, respectively).

Conclusions : Increases in pericardial fat after mADSCs injection were associated with the decreases in MI area and the increases in serum IL-10, thereby contributing to the improvement in left ventricular contractile function after AMI.