Abstract 13153: Long-Term Results of Cell-Free Biodegradable Scaffolds for In Situ Tissue-Engineering Pulmonary Artery in a Canine Model
We have previously developed a new biodegradable scaffold that does not require any cell seeding to create an in-situ tissue-engineering vasculature (iTEV) in a canine IVC model. We assessed the hypothesis that this scaffold can be applicable to a pulmonary position, as well (iTEV-PA). Animal experiments were conducted to test its characteristics and long-term efficacy. An 8-mm tubular biodegradable scaffold, consisting of polyglycolide knitted fibers and an L-lactide and ε-caprolactone copolymer sponge with outer glycolide and ε-caprolactone copolymer monofilament reinforcement, was implanted into the left pulmonary artery of 6 canines. All the animals remained alive without any major complications until euthanasia. The utility of the iTEV was evaluated from 6 to 12 months postoperatively. Angiography of the iTEV-PA after a year showed a well-formed vasculature without marked stenosis, aneurysmal change or thrombosis with an approximately 5mm in a diameter. Histological examinations revealed a well-formed vessel-like vasculature without calcification. However, the vascular smooth muscle cells were yet well developed at the year after implantation. Biochemical analyses showed no significant differences in the hydroxyproline and elastin contents compared with the native branch PA. In conclusion, the new scaffold can be useful for cell-free tissue-engineering of pulmonary artery. The long-term results revealed that the iTEV-PA was of good quality and had adapted its shape to the needs of the living body. Thus, this strategy of using biodegradable scaffold without cell seeding to create pulmonary artery would be applicable for pediatric cardiovascular surgery involving biocompatible materials.
- © 2012 by American Heart Association, Inc.