Abstract 12920: Glucosamine Improves Vascular Endothelial Function by Modulating Intracellular Redox Balance in Healthy Volunteers
Purpose: Glucosamine, a naturally occurring amino monosaccharide, has been shown to have anti-inflammatory and anti-atherosclerotic properties in experimental studies and used to treat osteoarthritis in humans. Recently, the vitamin and lifestyle cohort study has demonstrated that the use of glucosamine was significantly associated with decreased total mortality. Therefore, the purpose of this study is to investigate the effects of oral administration of glucosamine on vascular endothelial function and intracellular redox status.
Methods: Healthy male volunteers were enrolled in this study. In glucosamine group (n=44), glucosamine hydrochloride with 1500 (n=22) and 3000 mg/day (n=22) were administrated orally to them for 4-week observation period. Age-matched volunteers (n=21) without glucosamine administration for 4 weeks served as controls. Before and after observation, flow-mediated dilation (FMD) as a marker of vascular endothelial function, intraerythrocyte levels of GSH (the reduced form of glutathione) and GSSG (the oxidized form of glutathione), and blood atherosclerotic biochemical markers were measured.
Results: FMD significantly increased by low dose of glucosamine (from 6.3 ± 2.1 to 7.8 ± 2.6%, p<0.05) and high dose of glucosamine (from 6.8 ± 2.3 to 8.3 ± 2.7%, p<0.05). Low and high doses of glucosamine significantly increased intracellular GSH levels, respectively. Consequently, intracellular GSH/GSSG ratio as a marker of oxidative stress, significantly increased by low and high doses of glucosamine, respectively. Intracellular GSH levels significantly correlate with intracellular GSH/GSSG ratio (low and high doses group, r=0.53 and r=0.81, p<0.01). In high dose group, although intracellular GSH levels did not correlate with FMD, the changes in intracellular GSH levels by glucosamine significantly correlated with changes in FMD by glucosamine (r=0.48, p<0.05). In control group, any parameters did not change after observation.
Conclusions: The present study provides the first demonstration in humans that glucosamine improves vascular endothelial function by modulating intracellular redox balance. Thus, glucosamine may have anti-atherosclerotic properties through anti-oxidative action.
- © 2012 by American Heart Association, Inc.