Abstract 12213: Repeated Transendocardial Bonemarrow Cell Therapy Improves Diastolic Function in a Porcine Model of Chronic Ischemic Heart Disease
Introduction: Success of cell therapy is limited by low cell survival upon single cell injection leading to a temporary improvement in ejection fraction (EF).
Hypothesis:This study was designed to assess the functional effect of a novel priming strategy using two different bone marrow derived cell types via repeated transendocardial (TE) delivery in a porcine chronic myocardial infarction (MI) model compared to single cell injection.
Methods: Nineteen animals underwent repeated TE cell delivery using electromechanical mapping guidance at 4 and 8 weeks after MI. Animals received 107 autologous stem cells and were allocated to 3 groups: (1) placebo+placebo (2) mesenchymal stem cells (MSC)+placebo or (3) BMMNC+MSC delivery. Cardiac function was assessed by echocardiography, pressure-volume loops and intracoronary flow measurements. Myocardial biopsies were processed for collagen content and capillary density.
Results: Δ EF was significantly improved in cell treated groups compared to placebo treatment (Group 2 18±3%, Group 3 13±4% vs. Group 1 -9 ±3%; all P<0.01). However, no difference was observed i.
ΔEF (P=0.67) between the single and repetitive cell injected groups. Interestingly, the improvement in diastolic function was more pronounced after repeated cell injection assessed by a significant reduction in pressure half time (PHT), end-diastolic pressure (EDP) and diastolic stiffness (Eed) (see Fig 1). Moreover,repeated cell injection was associated with a greater decrease in coronary microvascular resistance and higher capillary density.
Conclusions: This study showed that cell injection improves systolic function in a chronic model of ischemic heart disease, moreover, repeated rather than a single injection further improved diastolic function.
- © 2012 by American Heart Association, Inc.