Abstract 11637: Remote Ischemic Preconditioning reduces Thrombus Formation in the Rat
Introduction: Remote ischemic preconditioning (RIPC) has been reported to reduce infarct size in patients with ST-elevation myocardial infarction. The mechanism is unknown but has been suggested to be protection against myocardial damage induced by reperfusion injury.
Hypothesis: RIPC has a direct effect on arterial thrombus formation and embolization in a dynamic in vivo rat model of experimental arterial thrombosis.
Methods: A thrombogenic arterial anastomosis was created in the femoral artery of 41 male Wistar rats. The rats were randomized to surgery with or without RIPC, which consisted of 10 minutes of arterial occlusion on the contralateral limb. The thrombus formation at the arteriotomy site was monitored with a HD video camera through the microscope during trans-illumination of the vessel after clamp release. The number of emboli was counted and thrombus area was measured every minute for 30 minutes. Data were Gaussian distributed and reported as mean +/- SEM. Students t-test was used for statistics.
Results: No significant differences in baseline characteristics between the two groups were found. The thrombus area (see figure 1) was significantly reduced in the group receiving RIPC (722 +/- 41 vs. 910 +/- 46, p = 0,0041) and number of emboli was significantly lower in RIPC rats compared to non-RIPC rats (0,88 +/- 0,06 vs. 1,08 +/- 0,06, p = 0,025).
Conclusions: RIPC leads to significantly reduced thrombus formation and reduces the number of emboli in this rat model. The mechanism of RIPC on thrombogenesis should be further explored. Our results might be clinically important in coronary thrombosis and cerebral thromboembolism, as reduction of thrombus formation and embolization will lead to attenuated ischemic damage. Figure 1: Thrombus area (number of pixels) displayed in relation to time after clamp removal in rats with and without remote preconditioning (RIPC). Mean and SEM values are shown. 1
- © 2012 by American Heart Association, Inc.