Abstract 11258: MicroRNA-150: A Novel Marker of Left Ventricular Function and Remodeling After Acute Myocardial Infarction
Purpose Prediction of left ventricular (LV) remodeling after acute myocardial infarction (AMI) is a challenging task. We hypothesized that circulating microRNAs (miRNAs) may predict LV remodeling after AMI.
Methods This study enrolled 90 patients with first ST elevation AMI. 60 patients characterized by echocardiography pre-discharge and at 6-months follow-up were the derivation cohort and 30 patients characterized by magnetic resonance imaging at 4-months follow-up were the validation cohort. Remodeling was defined as increase in LV end-diastolic volume between discharge and follow-up (ΔEDV >0). Circulating miRNAs in blood obtained at discharge were measured by microarrays and quantitative PCR. Potential predictors of remodeling were isolated using a systems-based approach.
Results Analysis of a network of interactions between miRNAs and genes known to regulate remodeling revealed strong association between miR-150 and remodeling. In the derivation cohort, miR-150 was detected only in the blood of patients without remodeling (ΔEDV ≤0). In the validation cohort, patients with remodeling had lower levels of miR-150 (2-fold, P=0.03). On multivariate analyses, miR-150 and N terminal-pro-brain natriuretic peptide (Nt-pro-BNP) were significant predictors of ΔEDV (P=0.007 and P=0.04, respectively). Receiver-operating characteristic (ROC) curves confirmed that miR-150 outperformed Nt-pro-BNP to predict remodeling (area under the ROC curve (AUC) of 0.74 and 0.60, P=0.02). In addition, we observed a significant association (P<0.001) between miR-150 and the change of ejection fraction between discharge and follow-up (ΔEF). miR-150 predicted ΔEF with an AUC of 0.87 whereas Nt-pro-BNP provided an AUC of 0.67.
Conclusion Low circulating levels of miR-150 are associated with the development of LV remodeling after AMI and may be used as prognostic biomarker.
- © 2012 by American Heart Association, Inc.