Abstract 11237: Adipocyte Progenitor Cell-derived Proinflammatory Cells Link Adipocyte Hyperplasia With Adipose Tissue Inflammation
In metabolic syndrome inflammation in obese visceral adipose tissue pivotally contribute to the development of cardiovascular and metabolic diseases. However, it is still unclear how inflammatory processes are initiated in obese adipose tissue. By screening genes potentially involved in adipogenesis using a shRNA library we identified RAD51, a DNA repair and recombination factor, as essential for the cell cycle progression coupled with adipocyte differentiation. Rad51+/- mice fed with a high-fat diet (HFD) exhibited reduced adipogenesis and were also protected from inflammation in visceral adipose tissue, suggesting a close link between adipocyte hyperplasia and adipose inflammation. Interestingly, during HFD-induced adipocyte hyperplasia adipocyte progenitor cells give rise to two populations: adipocytes and CD45+CD34+Sca1midCD24+ cells that recruit monocytes via chemokines. Rad51 haploinsufficiency suppressed proliferation of both adipocytes and CD45+CD34+Sca1midCD24+ cells, resulting in the suppression of the initial immune cell accumulation and following inflammatory processes. Our results clearly demonstrate that CD45+CD34+Sca1midCD24+ adipocyte progenitor-derived inflammatory (APDI) cells link obesity and initiation of inflammation in adipose tissue.
- © 2012 by American Heart Association, Inc.