Abstract 10964: Omega-3 Fatty Acid Supplementation Improves Endothelial Function and Endogenous Fibrinolysis in Cigarette Smokers
Introduction The effect of omega-3 fatty acids on endothelial function, fibrinolysis and platelet function remain uncertain. Hypothesis We hypothesized that omega-3 fatty acid supplementation would improve endothelial vasomotor function, endogenous fibrinolysis, and platelet and monocyte activation in healthy cigarette smokers; a group at increased risk of myocardial infarction.
Methods Twenty cigarette smokers were recruited into a randomised, double-blind, placebo-controlled, crossover trial of omega-3 fatty acid supplementation. Peripheral blood was taken for analysis of platelet and monocyte activation, and forearm blood flow was assessed in a subset of 12 smokers during intrabrachial infusions of acetylcholine, substance P and sodium nitroprusside. Stimulated plasma tissue plasminogen activator (t-PA) concentrations were measured during substance P infusion.
Results All vasodilators caused dose-dependent increases in forearm blood flow (P<0.0001). Compared to placebo, omega-3 fatty acid supplementation led to greater endothelium-dependent vasodilatation with acetylcholine and substance P (P=0.0032 and P=0.056). Substance P caused a dose-dependent increase in plasma t-PA concentrations (P<0.0001) that was greater after omega-3 fatty acid supplementation (P=0.029) [Figure]. Omega-3 fatty acids did not affect platelet-monocyte aggregation, platelet P-selectin or CD40L, or monocyte CD40.
Conclusion We have demonstrated for the first time that omega-3 fatty acids augment acute endothelial t-PA release and improve endothelial vasomotor function in cigarette smokers. Improved endogenous fibrinolysis and endothelial function may represent important mechanisms through which omega-3 fatty acids confer potential cardiovascular benefits.
- © 2012 by American Heart Association, Inc.